Rapid Charcoal Assay for Intrinsic Factor (IF), Gastric Juice Unsaturated B12 Binding Capacity, Antibody to IF, and Serum Unsaturated B12 Binding Capacity
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منابع مشابه
Rapid Charcoal Assay for Intrinsic Factor (if), Gastric Juice Unsaturated B12 Binding Capacity, Antibody to If, and Serum Unsaturated B12 Binding Capacity.
Bg CHESTER GorruEB, KAM-SENG LAU, Louis R. \VASSERMAN AND VICTOR HERBERT T HIS COMMUNICATION reports the use of protein-coated charcoal iii rapid assays for: (1) intrinsic factor (IF), (2) unsaturated B12 binding capacity of gastric juice, (3) antibody to IF, and (4) serum unsaturated B12 binding capacity. The assays are based on the finding that charcoal precoated with a large molecule can ads...
متن کاملA radioassay for serum B12 using unsaturated transcobalamin I as the B12 binding protein.
I N THE PAST SEVERAL YEARS a number of radioassay procedures for serum vitamin B12 have been described. Each method is based on the fact that unlabeled crystalline B12 will competitively inhibit isotopically labeled B12 (tracer B12) from binding to a specific B12 binding protein(s). Basic to each procedure is the reaction of B12 (labeled and unlabeled) with a limited quantity of binding protein...
متن کاملMeasurement of Serum Vitamin B12 Level Using Radioisotope Dilution and Coated Charcoal.
I N PREVIOUS REPORTS’3 it was shown that uncoated charcoal adsorbs both free vitamin B12 and B12 bound to proteins, but charcoal precoated by mixing with saturating quantities of albumin adsorbs only free B12. This finding has been confirmed.4 The charcoal particles may be considered as solid microsponges and the albumin coat as a molecular sieve surrounding each sponge. Small molecules such as...
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The reaction between binding intrinsic factor antibody and intrinsic factor-vitamin B(12) complex has been studied. Initially in the zone of antibody excess, the relationship between the amount of antigen present and the amount of antigen-antibody complex adsorbed onto zirconium phosphate gel was linear. With increasing amounts of antigen, the curve departed from linearity and reached a plateau...
متن کاملDetermination of the unsaturated iron-binding capacity of serum.
This paper deals with a modification of the methods suggested by Rath and Finch (1949) and Cartwright and Wintrobe (1949) for the determination of the unsaturated iron-binding capacity of the serum. Transport of iron by the blood plasma may be effected through union with a 8,/-globulin contained in the IV-7 fraction of Cohn (1945); this fraction has been called siderophyllin (Schade and Carolin...
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ژورنال
عنوان ژورنال: Blood
سال: 1965
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v25.6.875.875